Differential expression of osteopontin and bax, bcl-2 by fluoride / 中华预防医学杂志
Chinese Journal of Preventive Medicine
; (12): 107-110, 2005.
Article
in Zh
| WPRIM
| ID: wpr-299188
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the differential expression of bax, bcl-2 and osteopontin by fluoride in the renal tubular cells in vitro.</p><p><b>METHODS</b>The renal tubular cells were cultured and exposed to sodium fluoride (NaF) in 1, 5, 7.5, 12.5 mg F-/L level. The transcription level of bax, bcl-2 and osteopontin were investigated by reverse transcription - polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>The expression of bax mRNA in 7.5 and 12.5 mgF-/L groups (optical absorption ratio value was 2.37 +/- 0.18 and 2.64 +/- 0.19 respectively) was significantly increased (P < 0.01). On the contrary, the level of bcl-2 obviously decreased (5 mg F-/L group optical absorption ratio value, 0.80 +/- 0.22, P < 0.05; 7.5 mg F-/L group optical absorption ratio value 0.71 +/- 0.22, P < 0.01). The expression mRNA of osteopontin was significantly increased when cells were exposed to fluoride at 7.5 mg F-/L (optical absorption ratio value 2.01 +/- 0.40 P < 0.01), in that group the tubular cell apoptotic trend was obvious.</p><p><b>CONCLUSION</b>NaF might induce tubular cell apoptosis via activation of bax expression and bcl-2 suppression. Osteopontin might protect the tubule against apoptosis in a lower fluoride level, but the function should be decreased in higher fluoride level.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
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Sodium Fluoride
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RNA, Messenger
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Cells, Cultured
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Rats, Wistar
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Apoptosis
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Proto-Oncogene Proteins c-bcl-2
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Reverse Transcriptase Polymerase Chain Reaction
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Gene Expression Profiling
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Cell Biology
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Preventive Medicine
Year:
2005
Type:
Article