Detection of RNA of SARS coronavirus in hospital sewage / 中华预防医学杂志
Chinese Journal of Preventive Medicine
; (12): 257-260, 2004.
Article
in Zh
| WPRIM
| ID: wpr-299268
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>In order to explore the existence of SARS coronavirus (Co-V) and/or its RNA in sewage of hospitals administered SARS patients.</p><p><b>METHODS</b>A novel electropositive filter was used to concentrate the SARS-CoV from the sewage of two hospitals administered SARS patients in Beijing, including twelve 2,500 ml sewage samples from the hospitals before disinfection, and ten 25,000 ml samples after disinfection; as well as cell culture, RT-PCR and sequencing of gene to detect and identify the viruses from sewage.</p><p><b>RESULTS</b>There was no live SARS-CoV detected in the sewage in this study. The nucleic acid of SARS-CoV had been found in the 12 sewage samples before disinfection from both hospitals by semi-nested PCR. After disinfection, SARS-CoV RNA could only be detected from the samples from the 309th Hospital, and the others were negative.</p><p><b>CONCLUSION</b>It provides evidence that there is no live SARS-Cov in the sewage from hospitals with SARS patients though SARS-CoV RNA can be detected.</p>
Full text:
1
Index:
WPRIM
Main subject:
Sewage
/
Virology
/
RNA, Viral
/
Nucleocapsid
/
Reverse Transcriptase Polymerase Chain Reaction
/
Severe Acute Respiratory Syndrome
/
Severe acute respiratory syndrome-related coronavirus
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Genetics
/
Hospitals
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Preventive Medicine
Year:
2004
Type:
Article