Determination of polysaccharides content of Gentiana farreri from different producing areas based on anthrone-sulfuric acid method / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2774-2776, 2014.
Article
in Zh
| WPRIM
| ID: wpr-299856
Responsible library:
WPRO
ABSTRACT
Study a method for the detemination of the content of polysaccharides in Gentiana farreri, and analysis of the content of polysaccharides from different producing areas. The results showed that using the anthrone-sulfuric acid method, simple operation, accurate result. Sample was measured at 620 nm absorbance after anthrone-sulfuric acid color, at this wavelength, solution absorption and glucose showed a good linear relationship; The linearity was in the range of 0.01-0.07 g x L(-1) (r = 0.996 7). The recovery rate was 99.41%, with RSD of 2.0%. Considering the experimental conditions, to determine the solid-liquid ratio 1:60, extracting time 50 min, concentration of ethanol 80%. The mass fraction of polysaccharides was the highest to reached 0.743% in G. farreri from Gansu Xiahe. This experiment has laid a good foundation for further study on G. farreri.
Full text:
1
Index:
WPRIM
Main subject:
Polysaccharides
/
Sulfuric Acids
/
Time Factors
/
Linear Models
/
Chemistry
/
Chemistry Techniques, Analytical
/
Reproducibility of Results
/
Gentiana
/
Geography
/
Anthracenes
Type of study:
Prognostic_studies
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2014
Type:
Article