Quantitative determination of 5 active ingredients in different harvest periods of Ligusticum chuanxiong by HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1650-1655, 2014.
Article
in Zh
| WPRIM
| ID: wpr-300212
Responsible library:
WPRO
ABSTRACT
A simple and quick method is described for the determination of ferulic acid, senkyunolide I, senkyunolide H, senkyunolide A and ligustilide in rhizomes of Ligusticum chuanxiong. The 5 active ingredients in the sample was extracted using 40% ethanol and analyzed by reversed-phase high performance liquid chromatography (HPLC). Chromatography separation was performed using Agilent 1100 series HPLC system with a Symmetry C18 column and gradient elution with a mixture of three solvents : solvent A, acetonitrile, solvent B, methanol and solvent C, 1% aqueous acetic acid, 0 min to 5 min A: B: C 20: 40: 40, 5 min to 30 min A: B: C 60 to 100 : 0 : 40 to 0. The effluent was monitored using a VWD detector set at 321 nm (0-4.3 min) and 275 nm (4.31-30 min). The flow rate was set at 1 mL x min(-1) and the injection volume was 10 microL. The column temperature was maintained at 35 degrees C. The calibration curve was linear (r > or = 0.99) over the tested ranges. The average recovery was 94.44%-103.1% (n = 6). The method has been successfully applied to the analysis in different harvest periods of L. chuanxiong samples. In this paper, single-factor randomized block design to study the 5 components content of L. chuanxiong on ten collecting stages. For the L. chuanxiong collected from April 15th to May 30rd, the content of 5 ingredients increased primarily, and then decreased. Determine the appropriate harvest time has important significance to the promotion of the quality of L. chuanxiong.
Full text:
1
Index:
WPRIM
Main subject:
Acetonitriles
/
Solvents
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Time Factors
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Benzofurans
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4-Butyrolactone
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Chemistry
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Chromatography, High Pressure Liquid
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Acetic Acid
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Coumaric Acids
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Ligusticum
Type of study:
Clinical_trials
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2014
Type:
Article