Study on mRNA expression of fibronectin and integrin beta1 during fracture healing / 华西口腔医学杂志
West China Journal of Stomatology
; (6): 434-437, 2005.
Article
in Zh
| WPRIM
| ID: wpr-300275
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the spatial and temporal expression changes of fibronectin and integrin beta1 mRNA during fracture healing.</p><p><b>METHODS</b>Using in situ RT-PCR technique, the spatial and temporal expression pattern of both fibronectin and integrin beta1 mRNA was detected on paraffined slices in different rabbit mandibular fracture healing phases.</p><p><b>RESULTS</b>(1) The mRNA of integrin beta1 and fibronectin was widely expressed in fractured bone cells. The positive stain existed in cytoplasm and nucleolus. (2) After 7 days of fracture, increaseded integrin beta1 mRNA expression was observed, and it reached maximal levels approximately 14- 30 days post-fracture and returned to normal levels after 60 - 90 days. (3) Fibronectin mRNA was detected on 3 days post-fracture, it reached maximal levels at 7 - 14 days, faint expression was detected at 30 days, undetectable 60 days afterwards.</p><p><b>CONCLUSION</b>During fracture healing, the mRNA expression of integrin beta1 and fibronectin increased locally, FN and Itg beta1 cooperate with each other, it is suggested that they have an important influence on fracture healing.</p>
Full text:
1
Index:
WPRIM
Main subject:
RNA, Messenger
/
Fibronectins
/
Fracture Healing
/
Integrin beta1
/
Mandible
Limits:
Animals
Language:
Zh
Journal:
West China Journal of Stomatology
Year:
2005
Type:
Article