Screening for inhibitor of vascular endothelial growth factor from random peptide library / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 540-543, 2002.
Article
in Zh
| WPRIM
| ID: wpr-301941
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To screen for the inhibitor of vascular endothelial growth factor (VEGF) 165 from random peptide library.</p><p><b>METHODS</b>Positive phage clones were rescued after two rounds of panning and competitive elution. Its affinity activity to KDR was monitored through ELISA, immunohistochemical method, Chicken CAM assay and MTT.</p><p><b>RESULTS</b>Five specific binding positive target molecule phage clones were obtained which were able to bind to cells whose surface had high KDR, among which, clone 3 and 13 could effectively block the vascularization of the chorioallantoic membrane of chick embryo, but they were not inhibitive on the proliferation of high KDR expression cells.</p><p><b>CONCLUSION</b>The peptides, being the inhibitors of VEGF, may be useful in the treatment of cancers.</p>
Full text:
1
Index:
WPRIM
Main subject:
Peptides
/
Pharmacology
/
Binding Sites
/
Enzyme-Linked Immunosorbent Assay
/
Endothelial Growth Factors
/
Lymphokines
/
Peptide Library
/
Intercellular Signaling Peptides and Proteins
/
Vascular Endothelial Growth Factors
/
Vascular Endothelial Growth Factor A
Type of study:
Clinical_trials
/
Diagnostic_studies
/
Screening_studies
Limits:
Animals
/
Humans
Language:
Zh
Journal:
Chinese Journal of Oncology
Year:
2002
Type:
Article