Effect of NF-kappaB on the induction of PDGF-B transcription by angiotensin II in the ECV304 cell line / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 433-438, 2002.
Article
in En
| WPRIM
| ID: wpr-302307
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To examine the effect of angiotensin II (Ang II) on nuclear factor-kappa B (NF-kappaB) activation in human endothelial cell line ECV304 and the molecular mechanism by which Ang II activates NF-kappaB.</p><p><b>METHODS</b>ECV304 cells were transiently cotransfected with an NF-kappaB/luciferase reporter gene and inactive NF-kappaB-inducing kinase (NIK), IkappaB kinase alpha (IKK(alpha)), IkappaB kinase beta (IKK(beta)) mutants or vectors, respectively. The effect on NF-kappaB was detected by using an electrophoretic mobility shift assay (EMSA) and overexpression of the mutants enabled blocking of reporter gene activation induced by Ang II. With immunofluorescence and immuno-electronic microscope techniques, including confocal microscopy and gold particle labeled electronic microscopy, definite cytoplasmic-to-nuclear translocations of NF-kappaB activation were detected using subunits p50 and p65 induced by Ang II.</p><p><b>RESULTS</b>The translocation of p50 in nuclei was highly remarkable 2 hours after Ang II stimulation, and the activity was somewhat reduced 6 hours after stimulation to the 18th hour. Northern blot also showed PDGF-B mRNA increased by stimulation of Ang II for 18 hours.</p><p><b>CONCLUSION</b>Ang II is effective in stimulating NF-kappaB activation through a pathway dependent on NIK, IKK(alpha) and IKK(beta), and induces PDGF-B transcription in the endothelial cell line, ECV304.v</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Transcription, Genetic
/
Angiotensin II
/
Endothelium, Vascular
/
Cell Line
/
NF-kappa B
/
Proto-Oncogene Proteins c-sis
/
Electrophoretic Mobility Shift Assay
/
Genetics
/
Metabolism
Limits:
Humans
Language:
En
Journal:
Chinese Medical Journal
Year:
2002
Type:
Article