Mechanisms of sodium ferulate inhibition of collagen synthesis in hepatic stellate cells / 药学学报
Acta Pharmaceutica Sinica
;
(12): 577-580, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-302759
ABSTRACT
<p><b>AIM</b>To study the mechanisms of sodium ferulate (SF) on inhibition of collagen synthesis in hepatic stellate cells.</p><p><b>METHODS</b>Collagen synthesis was analyzed by measuring 3H-proline incorporation. ELISA method was used to study the effect of SF on transforming growth factor beta1 (TGFbeta1) level in cultured HSC-T6 cell. The effect of SFon the TGFbeta1 activity in the supernatant of culture was analyzed by mink lung epithelial cell (Mv1Lu) proliferation inhibition by MTT assay.</p><p><b>RESULTS</b>SF inhibited collagen synthesis in hepatic stellate cells stimulated with TGFbeta1. SF was shown to decrease TGFbeta1 level in the supernatant of HSC-T6 increased by oxidative stress. TGFbeta1 activity was intervened by SF.</p><p><b>CONCLUSION</b>SF could decrease collagen synthesis, with mechanism may be associated with that SF intervened TGFbeta1 activity, and reduced the level of TGFbeta1 increased by oxidative stress.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Cells, Cultured
/
Transforming Growth Factor beta
/
Collagen
/
Oxidative Stress
/
Coumaric Acids
/
Hepatocytes
/
Cell Biology
/
Cell Proliferation
/
Epithelial Cells
Limits:
Animals
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2004
Type:
Article
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