Identification of the major allergen of Macrobrachium rosenbergii (giant freshwater prawn)
Asian Pacific Journal of Tropical Biomedicine
;
(12): 50-54, 2012.
Article
in English
| WPRIM
| ID: wpr-303625
ABSTRACT
<p><b>OBJECTIVE</b>To characterize the major allergens of Macrobrachium rosenbergii (giant freshwater prawn).</p><p><b>METHODS</b>Raw and cooked extracts of the giant freshwater prawn were prepared. The IgE reactivity pattern was identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting technique with the sera of 20 skin prick test (SPT) positive patients. The major allergen identified was then characterized using the proteomics approach involving a combination of two-dimensional (2-DE) electrophoresis, mass spectrometry and bioinformatics tools.</p><p><b>RESULTS</b>SDS-PAGE of the raw extract showed 23 protein bands (15-250 kDa) but those ranging from 40 to 100 kDa were not found in the cooked extract. From immunoblotting experiments, raw and cooked extracts demonstrated 11 and 5 IgE-binding proteins, respectively, with a molecular mass ranging from 15 to 155 kDa. A heat-resistant 36 kDa protein was identified as the major allergen of both extracts. In addition, a 42 kDa heat-sensitive protein was shown to be a major allergen of the raw extract. The 2-DE gel fractionated the prawn proteins to more than 50 different protein spots. Of these, 10 spots showed specific IgE reactivity with patients' sera. Matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) analysis led to identification of 2 important allergens, tropomyosin and arginine kinase.</p><p><b>CONCLUSIONS</b>It can be concluded that the availability of such allergens would help in component-based diagnosis and therapy of prawn allergies.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Mass Spectrometry
/
Immunoglobulin E
/
Allergens
/
Electrophoresis, Gel, Two-Dimensional
/
Immunoblotting
/
Chemistry
/
Computational Biology
/
Palaemonidae
/
Electrophoresis, Polyacrylamide Gel
/
Allergy and Immunology
Limits:
Animals
/
Humans
Language:
English
Journal:
Asian Pacific Journal of Tropical Biomedicine
Year:
2012
Type:
Article
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