Differentiation of porcine amniotic fluid stem cells into the beating cardiomyocytes / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1206-1214, 2011.
Article
in Zh
| WPRIM
| ID: wpr-304584
Responsible library:
WPRO
ABSTRACT
The aim of this research is to find an effective cardiomyocyte-induced method derived from porcine amniotic fluid stem cells (pAFS). For cardiac differentiation, the cells were formed embryoid bodies (EBs) firstly, then cultured in induced-medium including 5-azacytidine (5-aza) and vitamin C (Vc). We detected the specific markers of cardiomyocyte by immunocytochemistry, RT-PCR and transmission electron microscope. The results showed that some embryoid bodies beat rhythmically after 10 days of induction. Furthermore, analysis of t test revealed that the percentage of beating cardiomyocyte-like cell clusters was highest (33%) when induction using 0.1 mmol/L Vc and 5 micromol/L 5-aza. Immunocytochemistry analysis demonstrated that cardiomyocyte-like cell clusters expressed alpha-actin, Tnni3. RT-PCR analysis also illustrated that TbX5, Gata4, alpha-MHC and Tnni3 were expressed positive in cardiomyocyte-like cell clusters. Especially, we observed basic structures of myocardium, such as myofilament, glycogen granule and so on by transmission electron microscope. In conclusion, 5-azacytidine and vitamin C could promote differentiation of pAFS into myocardium.
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Ascorbic Acid
/
Azacitidine
/
Swine
/
Cell Differentiation
/
Cells, Cultured
/
Cell Biology
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Myocytes, Cardiac
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Embryonic Stem Cells
/
Embryoid Bodies
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2011
Type:
Article