Isolation and identification of Japanese encephalitis virus in Liaoning Province / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 61-65, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-305544
ABSTRACT
<p><b>BACKGROUND</b>To study arboviruses carried by mosquitoes in Liaoning Province in 2002.</p><p><b>METHODS</b>Totally 4927 mosquitoes were collected from Liaoning Province in July 2002. Virus strains were isolated by inoculating BHK-21, C6/36 and Vero cells. The newly isolated strains were identified by serological (ELISA and IFA) and molecular methods (Real-Time PCR and RT-PCR).</p><p><b>RESULTS</b>Two strains were isolated from mosquitoes causing cytopathogenic effect (CPE) on cells and were fatal to suckling mice. Serological tests showed that both were positive for the antibody to JEV. The PrM and E gene were cloned and sequenced. The phylogenetic analysis showed that the new isolates belonged to genotype I, JEV. Sequence analysis showed that the homology of nucleotide sequences and amino acid (AA) sequences between the two strains was 100%. Compared with the nucleotide sequences between the two strains and the standard JEV vaccine strain SA14-14-2, the difference was up to 4.11%, and the difference of AA was 0.6%.</p><p><b>CONCLUSION</b>Two strains of JEV were isolated and identified in Liaoning province, both belonged to genotype I JEV.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phylogeny
/
Virology
/
Vero Cells
/
Cell Line
/
Chlorocebus aethiops
/
China
/
Viral Envelope Proteins
/
Classification
/
Sequence Analysis, DNA
/
Encephalitis Virus, Japanese
Type of study:
Diagnostic study
Limits:
Animals
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2006
Type:
Article
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