Expression of Id1 and Id3 in endometrial carcinoma and their roles in regulating biological behaviors of endometrial carcinoma cells in vitro / 南方医科大学学报
Journal of Southern Medical University
; (12): 812-818, 2013.
Article
in Zh
| WPRIM
| ID: wpr-306462
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of inhibitor of DNA differentiation/DNA binding 1 (Id1) and Id3 in endometrial carcinoma and explore their roles in regulating the proliferation, invasion, migration and adhesion of endometrial carcinoma cells in vitro.</p><p><b>METHODS</b>Id1 and Id3 expression in 4 fresh endometrial cancer tissue specimens and matched adjacent tissues were detected using Western blotting. Two endometrial cancer cell lines, HEC-1-B and RL-952, were both divided into 4 groups, namely the untreated group, blank virus group, promoter group and Id1/Id3 double-knockdown group, and their expressions of MMP2, CXCR4 and P21 were detected by qRT-PCR and Western blotting. The proliferation, invasion, migration and adhesion of the cells were evaluated with MTT, Transwell, wound-healing, and adhesion assays.</p><p><b>RESULTS</b>Endometrial carcinoma tissues showed significantly higher Id1 and Id3 expression than the adjacent tissues (P<0.05). In the two endometrial carcinoma cell lines, Id1/Id3 double-knockdown significantly decreased MMP2 and CXCR4 expression and increased P21 expression at both mRNA and protein levels (P<0.05), and resulted in suppressed cell proliferation, invasion, migration and adhesion.</p><p><b>CONCLUSION</b>Id1 and Id3 expressions are up-regulated in endometrial carcinoma to promote the proliferation, invasion, migration and adhesion of the tumor cells by increasing MMP2 and CXCR4 expression and reducing P21 expression. Therapies targeting Id1/Id3 can be a novel strategy for treatment of endometrial carcinoma.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Cell Adhesion
/
Cell Movement
/
Proto-Oncogene Proteins p21(ras)
/
Endometrial Neoplasms
/
Receptors, CXCR4
/
Matrix Metalloproteinase 2
/
RNA Interference
/
Cell Line, Tumor
/
Cell Proliferation
Limits:
Female
/
Humans
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2013
Type:
Article