Immunization with dendritic cells infected with human AFP adenovirus vector effectively elicits immunity against mouse hepatocellular carcinomas / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 13-16, 2006.
Article
in Zh
| WPRIM
| ID: wpr-308433
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of dendritic cells (DCs) infected with adenovirus vector encoding xenogeneic alpha-fetoprotein (AFP) on breaking the immune tolerance and induction of immunity against hepatocellular carcinomas.</p><p><b>METHODS</b>Human and mouse alpha-fetoprotein full-length cDNA were cloned from human HepG2 and mouse Hepa 1 - 6 hepatoma cell lines, respectively, using RT-PCR, and then inserted into adenoviral shuttle vectors to construct Ad hAFP and Ad mAFP. Mice were immunized with Ad hAFP-infected DC and in vitro CTL activity against Hepa 1 - 6 cells was examined by standard (51)Cr release assay. Survival was studied of the immunized mice, with or without depletion of CD8+ or CD4+ T cells, inoculated with Hepa 1 - 6 mouse hepatoma cells.</p><p><b>RESULTS</b>The lytic activity of CTL elicited by the Ad hAFP-infected DCs were much stronger than that by Ad mAFP-infected DCs. 80% of the Ad hAFP/DCs-immunized mice of the inoculated with 5 x 10(6) Hepa 1 - 6 hepatoma cells were still alive two months after inoculation. However, the Ad mAFP/DCs-immunized mice inoculated with 1 x 10(6) Hepa 1 - 6 cells were just 20% surviving two months later. Depletion of CD8+ or CD4+ T cells abolished such an antigen-specific immunity elicited by the DCs infected with Ad hAFP.</p><p><b>CONCLUSION</b>Adenovirus vector-mediated xenogeneic AFP-infected DCs can effectively break the immune tolerance to hepatocellular carcinomas in an animal model and induce strong antigen-specific T cell response, which are dependent on CD8+ and CD4+ T cells.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Dendritic Cells
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CD4-Positive T-Lymphocytes
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Alpha-Fetoproteins
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Adenoviridae
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Immunization
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Carcinoma, Hepatocellular
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CD8-Positive T-Lymphocytes
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Cytotoxicity, Immunologic
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Cell Line, Tumor
Limits:
Animals
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Female
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Humans
Language:
Zh
Journal:
Chinese Journal of Oncology
Year:
2006
Type:
Article