Effects of TNF-α on ICAM-1 and LFA-1 expression in peripheral blood mononuclear cells of children with febrile seizures / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
; (12): 285-287, 2011.
Article
in Zh
| WPRIM
| ID: wpr-308810
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of TNF-α on ICAM-1 and LFA-1 expression in peripheral blood mononuclear cells (PBMC) of children with febrile seizures (FS).</p><p><b>METHODS</b>Sixteen children with FS and 16 age- and gender-matched healthy children were enrolled. The samples of PBMC from FS children were randomized into two groups with or without TNF-α treatment (TNF-α concentration 1.0 ng/mL). PBMC were purified and cultured with a conventional method in vitro. The expression of ICAM-1 and LFA-1 in PBMC was determined by flow cytometry (FCM).</p><p><b>RESULTS</b>ICAM-1[(20±9)% vs (14±7)%)]and LFA-1[(43±16)% vs (30±16)%]expression in PBMC in the untreated FS group was significantly higher than that in the normal control group (P<0.05). Compared with the untreated FS group, the treatment with TNF-α remarkably increased the ICAM-1 expression[(27±11)%](P<0.05). PBMC LFA-1 expression[(52±21)%]in the TNF-α-treated group was higher than that in the untreated FS group, although there were no statistical differences between the two groups.</p><p><b>CONCLUSIONS</b>TNF-α treatment may increase LFA-1 and ICAM-1 expression in PBMC of children with FS.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Blood
/
Leukocytes, Mononuclear
/
Chemistry
/
Lymphocyte Function-Associated Antigen-1
/
Tumor Necrosis Factor-alpha
/
Intercellular Adhesion Molecule-1
/
Seizures, Febrile
/
Allergy and Immunology
Type of study:
Clinical_trials
Limits:
Child
/
Child, preschool
/
Female
/
Humans
/
Infant
/
Male
Language:
Zh
Journal:
Chinese Journal of Contemporary Pediatrics
Year:
2011
Type:
Article