Effect of Betamethasone on the cAMP of olfactory receptor neurons in rat / 中华耳鼻咽喉头颈外科杂志
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
; (12): 770-773, 2007.
Article
in Zh
| WPRIM
| ID: wpr-309437
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of Betamethasone on the cyclic adenosine monophosphate (cAMP) in olfactory receptor neurons (ORNs) and explore the possible mechanisms of the recovery of olfactory disorders by steroid treatment</p><p><b>METHODS</b>ORNs membrane was extracted and incubated with 0.1 mg/ml and 1.0 mg/ml Betamethasone. The concentrations of cAMP were measured by enzyme-linked immunosorbent assay at different times.</p><p><b>RESULTS</b>Compared with the control group, all Betamethasone groups showed differences, indicating 0.1 mg/ml (P < 0.05) and 1.0 mg/ml (P < 0.01) Betamethasone could rise the concentration of cAMP. The Betamethasone had obvious effects on cAMP production in rat ORNs and there was a dose-dependent effect. There was no difference between 5 minutes groups and 30 minutes groups.</p><p><b>CONCLUSIONS</b>Steroid hormone could enhance the production of cAMP of ORNs. Steroid hormone may thus contribute to the recovery of olfactory disorders partially, at least, through the effect on AC-cAMP in olfactory transduction.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
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Betamethasone
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Signal Transduction
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Rats, Wistar
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Olfactory Receptor Neurons
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Cyclic AMP
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Patch-Clamp Techniques
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Membrane Potentials
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Metabolism
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
Year:
2007
Type:
Article