Effect of danshensu on activation of JNK pathway in hepatic stellate cells(HSCs) induced by IL-1 beta / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 451-454, 2009.
Article
in Zh
| WPRIM
| ID: wpr-310062
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVES</b>To investigate the inhibitory effect of danshensu on the activation of JNK signaling in rat hepatic stellate cells (HSCs) induced by IL-1beta.</p><p><b>METHODS</b>The proliferation of primary rat HSCs treated with different concentration of Danshensu was checked by MTT colorimetric assay. The expression and phosphorylation of JNK and P-JNK was detected by western blotting. Synthesis and secretion of collagen I were detected by the quantitative immunocytochemical assay and ELISA.</p><p><b>RESULTS</b>Danshensu inhibited the proliferation of HSCs in a dose-dependent manner. At the concentration of 0.0625 to 0.25 mmol/L, Danshensu significantly repressed the proliferation of HSC induced by IL-1beta (P < 0.05). Synthesis and secretion of Type I collagen was significantly decreased 24 hours after 0.25 mmol/L Danshensu treatment (P < 0.01). The phosphorylation of JNK induced by IL-1beta was significantly inhibited by Danshensu treatment (P < 0.05), however, the expression of JNK was not regulated by Danshensu.</p><p><b>CONCLUSION</b>Danshensu represses the activation and proliferation of HSCs, and inhibits the synthesis and secretion of Type I collagen, possibly via the repression of the JNK signal transduction.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
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Immunohistochemistry
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Signal Transduction
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Down-Regulation
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Cells, Cultured
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Blotting, Western
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Rats, Wistar
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Bodily Secretions
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Mitogen-Activated Protein Kinases
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Collagen Type I
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Hepatology
Year:
2009
Type:
Article