Application of atomic force microscopy (AFM) to study bacterial biofilms / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1399-1410, 2017.
Article
in Zh
| WPRIM
| ID: wpr-310583
Responsible library:
WPRO
ABSTRACT
Because of the nanometre resolution, piconewton force sensitivity, label-free technique and the ability to operate in liquid environments, atomic force microscopy (AFM) has emerged as a powerful tool to explore the biofilm development processes. AFM provides three-dimensional topography and structural details of biofilm surfaces under in-situ conditions. It also helps to generate key information on the mechanical properties of biofilm surfaces, such as elasticity and stickiness. Additionally, single-molecule and single-cell force spectroscopies can be applied to measure the strength of adhesion, attraction, and repulsion forces between cell-solid and cell-cell surfaces. This paper outlined the basic principle of AFM technique and introduced recent advances in the application of AFM for the investigation of ultra-morphological, mechanical and interactive properties of biofilms. Furthermore, the existing problems and future prospects were discussed.
Full text:
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2017
Type:
Article