Differential Proteomics Reveals the Potential Injury Mechanism Induced by Heavy Ion Radiation in Mice Ovaries / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 301-307, 2017.
Article
in En
| WPRIM
| ID: wpr-311410
Responsible library:
WPRO
ABSTRACT
In the present study, we used a proteomics approach based on a two-dimensional electrophoresis (2-DE) reference map to investigate protein expression in the ovarian tissues of pubertal Swiss-Webster mice subjected to carbon ion radiation (CIR). Among the identified proteins, ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) is associated with the cell cycle[1] and that it influences proliferation in ovarian tissues. We analyzed the expression of UCH-L1 and the proliferation marker proliferation cell nuclear antigen (PCNA) following CIR using immunoblotting and immunofluorescence. The proteomics and biochemical results provide insight into the underlying mechanisms of CIR toxicity in ovarian tissues.
Full text:
1
Index:
WPRIM
Main subject:
Ovary
/
Radiation Effects
/
Biomarkers
/
Electrophoresis, Gel, Two-Dimensional
/
Carrier Proteins
/
Random Allocation
/
Gene Expression
/
Proteomics
/
Ubiquitin Thiolesterase
/
Heavy Ion Radiotherapy
Limits:
Animals
Language:
En
Journal:
Biomedical and Environmental Sciences
Year:
2017
Type:
Article