Inhibiting severe acute respiratory syndrome-associated coronavirus by small interfering RNA / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 1262-1264, 2003.
Article
in En
| WPRIM
| ID: wpr-311704
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effectiveness of small interfering RNA (siRNA) on inhibiting severe acute respiratory syndrome (SARS)-associated coronavirus replication, and to lay bases for the future clinical application of siRNA for the treatment of viral infectious diseases.</p><p><b>METHODS</b>Vero-E6 cells was transfected with siRNA before SARS virus infection, and the effectiveness of siRNA interference was evaluated by observing the cytopathic effect (CPE) on Vero-E6 cells.</p><p><b>RESULTS</b>Five pairs of siRNA showed ability to reduce CPE dose dependently, and two of them had the best effect.</p><p><b>CONCLUSION</b>siRNA may be effective in inhibiting SARS-associated coronavirus replication.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Virus Replication
/
Vero Cells
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Transfection
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Chlorocebus aethiops
/
RNA, Small Interfering
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Severe acute respiratory syndrome-related coronavirus
Limits:
Animals
Language:
En
Journal:
Chinese Medical Journal
Year:
2003
Type:
Article