Study of the anti-endotoxin effect of beta-1, 2, 3, 4, 6-penta-O-galloyl-D-glucopyranose in vitro / 中华烧伤杂志
Chinese Journal of Burns
;
(6): 282-284, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-312557
ABSTRACT
<p><b>OBJECTIVE</b>To study the anti-endotoxin effect of beta-1, 2, 3, 4, 6-penta-O-galloyl-D-glucopyranose (PGG) in vitro.</p><p><b>METHODS</b>The affinity of PGG with lipid A was determined with biosensor technology, and the endotoxin-neutralizing effect was assayed with LAL. Human peripheral blood mononuclear cells (hPBMC) were separated from healthy donors and cultured in vitro. The effect of different concentrations of PGG on the release of TNF-alpha and hIL-6 from LPS-stimulated hPBMC were measured by ELISA method.</p><p><b>RESULTS</b>Lipid A was combined with different concentrations of PGG. The combination speed was shortened with the increase in PGG concentration. The KD value between PGG and Lipid A was 5.2 x 10(-7) mol/L. The release of TNF-alpha and IL-6 of hPBMC under LPS stimulation (958 +/- 234 ng/L vs 1 351 +/- 99 ng/L) was obviously inhibited by PGG in the concentration of higher than 20 mg/L compared with that without PGG treatment (1 788 +/- 171 ng/L vs 1 965 +/- 232 ng/L, P < 0.05).</p><p><b>CONCLUSION</b>PGG show an anti-endotoxin effect in vitro, which may be associated with its ability to combine and neutralize endotoxin.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
In Vitro Techniques
/
Leukocytes, Mononuclear
/
Biosensing Techniques
/
Cells, Cultured
/
Interleukin-6
/
Tumor Necrosis Factor-alpha
/
Hydrolyzable Tannins
/
Dose-Response Relationship, Drug
/
Drug Antagonism
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Burns
Year:
2005
Type:
Article
Similar
MEDLINE
...
LILACS
LIS