Association of C677T gene polymorphisms of methylenetetrahydrofolate reductase and plasma homocysteine level with hyperlipidemia / 南方医科大学学报
Journal of Southern Medical University
; (12): 1195-1198, 2014.
Article
in Zh
| WPRIM
| ID: wpr-312608
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the association of methylenetetrahydrofolate reductase (MTHFR) gene C677T mutation and plasma homocysteine (Hcy) levels with hyperlipidemia.</p><p><b>METHODS</b>Blood samples were collected from 1591 adults for detecting MTHFR gene C677T polymorphism with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), plasma Hcy levels with enzymatic cycling method, and blood lipid levels as well. The patients were divided according to the lipid levels into hyperlipidemia group (n=694) and healthy control group (n=897) and the differences in MTHFR gene C677T polymorphisms and plasma Hcy levels were compared.</p><p><b>RESULTS</b>The hyperlipidemia group and healthy control group showed no significant differences in CC, CT, or TT genotype frequencies or C and T allele frequencies of MTHFR C677T gene, and had comparable plasma Hcy levels (P>0.05). Patients with 3 different MTHFR C677T genotypes had significant differences in plasma Hcy levels (P<0.01) but not in blood lipid levels (P>0.05). Pairwise comparison indicated a significantly higher plasma Hcy level in TT genotype than in CC and CT genotypes (P<0.01), and the latter two genotypes showed no significant difference (P>0.05).</p><p><b>CONCLUSION</b>MTHFR C677T polymorphisms and plasma Hcy levels are closely related but neither of them is associated with hyperlipidemia. The TT genotype is associated with a significantly higher plasma Hcy level than CC and CT genotypes.</p>
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Index:
WPRIM
Main subject:
Polymorphism, Genetic
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Blood
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Polymorphism, Restriction Fragment Length
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Polymerase Chain Reaction
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Methylenetetrahydrofolate Reductase (NADPH2)
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Gene Frequency
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Genetics
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Genotype
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Homocysteine
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Hyperlipidemias
Limits:
Adult
/
Humans
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2014
Type:
Article