Study on the automatic ribotyping for Enterobacter sakazakii / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 900-902, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-316094
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the ribotyping fingerprint of Enterobacter sakazakii (E. sakazakii) isolated from food and its typing power.</p><p><b>METHODS</b>Two standard strains and twenty-eight isolates of E.sakazakii were analyzed by the DuPont Riboprinter(TM) microbial characterization system. The relevant database was established and the fingerprint patterns were analyzed with BioNumerics software.</p><p><b>RESULTS</b>This system grouped two standard strains and twenty-eight E.sakazakii isolates into 26 ribotypes, and four ribotypes included two strains respectively, the other twenty-two strains showed different ribotypes. The lowest similarity was 31.58%. The number of bands by ribotyping was approximately ten and the molecular weight of these bands ranged from 1 to 50 kb. By the clustering program in BioNumerics, these isolates could be grouped into four clusters.</p><p><b>CONCLUSION</b>The automatic ribotyping method is convenient and fast in E.sakazakii typing.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
DNA, Bacterial
/
DNA, Ribosomal
/
Classification
/
Ribotyping
/
Cronobacter sakazakii
/
Food Microbiology
/
Genetics
/
Methods
Language:
Chinese
Journal:
Chinese Journal of Preventive Medicine
Year:
2009
Type:
Article
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