Quantitative microarray-based DNA methylation analysis of E-cadherin gene promoter in acute leukemia / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 41-44, 2007.
Article
in Zh
| WPRIM
| ID: wpr-316249
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To quantitatively detect the methylation of E-cadherin gene 5'-CpG islands in acute leukemia by microarray-based DNA analysis and to briefly discuss the role of microarry for detection of methylation in tumors.</p><p><b>METHODS</b>Bisulfite-modified DNA was used as a template for PCR amplification, resulting in conversion of unmethylated cytosine, but not methylated cytosine, into thymine within CpG islands of interest. Five sets of oligonucleotide probes were designed to fabricate a DNA microarray to detect the methylation changes of E-cadherin gene CpG islands in acute leukemia. By drawing a standard curve to assess the levels of changes in methylation detected in the examined samples.</p><p><b>RESULTS</b>Microarray assay was successfully used to quantitatively detect methylation changes of E-cadherin gene in 5 acute leukemia samples. Varying degree of methylation was detected in five regions and the hypermethylation region was the same. The result was validated by gene sequencing.</p><p><b>CONCLUSION</b>Microarray assay may be applied as an useful tool for mapping methylation changes in multiple CpG loci and for leukemia research. It is more time-saving and labor-saving than gene sequencing and can be used to quantitatively detect changes in methylation with high throughput.</p>
Full text:
1
Index:
WPRIM
Main subject:
Molecular Sequence Data
/
Base Sequence
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Leukemia, Myeloid, Acute
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Cadherins
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Promoter Regions, Genetic
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CpG Islands
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DNA Methylation
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Oligonucleotide Array Sequence Analysis
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Precursor Cell Lymphoblastic Leukemia-Lymphoma
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Genetics
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Oncology
Year:
2007
Type:
Article