Construction of a eukaryotic expression plasmid of Humanin / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
;
(12): 11-13, 2005.
Article
in English
| WPRIM
| ID: wpr-316385
ABSTRACT
<p><b>OBJECTIVE</b>To construct a eukaryotic expression plasmid pcDNA3.1(-)-Humanin.</p><p><b>METHODS</b>The recombinant plasmid pGEMEX-1-Humanin was digested with restriction endonucleases BamH I and Hind III and the Humanin gene fragments, about 100 bp length, were obtained. Then the Humanin gene fragments were inserted into eukaryotic expression vector pcDNA3.1(-) and the recombinant plasmids pcDNA3.1(-)-Humanin were identified by sequencing.</p><p><b>RESULTS</b>Recombinant plasmid DNA successfully produced a band which had the same size as that of the Humanin positive control. The sequence of recombinant plasmids accorded with the Humnain gene sequence.</p><p><b>CONCLUSIONS</b>A eukaryotic expression plasmid of Humanin was successfully constructed.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Recombinant Proteins
/
Molecular Sequence Data
/
Base Sequence
/
Protein Engineering
/
Proteins
/
Chemistry
/
Cloning, Molecular
/
Intracellular Signaling Peptides and Proteins
/
Escherichia coli
Limits:
Humans
Language:
English
Journal:
Journal of Zhejiang University. Science. B
Year:
2005
Type:
Article
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