Preparation and identification of the monoclonal antibodies against programmed death-1 ligant 1 / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 380-382, 2010.
Article
in Zh
| WPRIM
| ID: wpr-316892
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To prepare monoclonal antibodies (McAbs) against human programmed death-1 ligant 1(PD-L1) and identify its bioactivity.</p><p><b>METHODS</b>We immunized the BALB/c mice with Thioredoxin-(PD-L1) recombination protein which expressed by prokaryotic system. Prepare hybridoma cell by hybridoma technology and used enzyme-linked immunosorbent assay(ELISA) and Western-blotting assays to select positive hybridoma identify cell. Competition inhibition ELISA was carried out to identify the special bioactivity of antibody.</p><p><b>RESULTS</b>4 hybridoma cell strains which could secrete anti-(PD-L1) antibodies stably were selected. The McAbs has good affinity with its receptor. Purify anti-(PD-L1) with title 132 000 was obtained after large quantity preparation. At the same time we obtained 1 cell stain which could secret special anti-Trx McAbs.</p><p><b>CONCLUSIONS</b>We obtained anti-(PD-L1) McAbs with good bioactivity successfully, which lay the foundation for further study.</p>
Full text:
1
Index:
WPRIM
Main subject:
Enzyme-Linked Immunosorbent Assay
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Antigens, CD
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Allergy and Immunology
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B7-H1 Antigen
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Hybridomas
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Mice, Inbred BALB C
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Antibodies, Monoclonal
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Antibody Specificity
Limits:
Animals
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Female
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Humans
Language:
Zh
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2010
Type:
Article