Your browser doesn't support javascript.
loading
Soluble expression of A/H1N1 influenza virus HA with Drosophila S2 cell line and its bio-activity identification / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology ; (6): 360-362, 2013.
Article in Chinese | WPRIM | ID: wpr-318020
ABSTRACT
<p><b>OBJECTIVE</b>To express soluble HA of A/H1N1 influenza virus in drosophila S2 cell line and identify its bio-activity.</p><p><b>METHODS</b>HA gene was amplified from A/Shenzhen/71/09 virus strain using RT-PCR, then we constructed pAC5.1-HA expression vector, which was co-transfected into S2 cell with pCoblast vector. After transfection, stable S2 cell was selected through Blasticindin. HA in the supernatant was identified with Western Blot assay and purified with Ni-column. Recombinant HA was immunized into BALB/c mice 3 times, and the Abs titers were evaluated with ELISA.</p><p><b>RESULTS</b>We successfully cloned HA gene with 1.7 x 10(3) bp of A/Shenzhen/71/09 virus strain and got recombinant pAC5. 1-HA expression vector. Stable S2 cell line was established after transfection and selection, which continuously expressed HA with molecular weight 75 x 10(3) D. After immunization with HA, the Abs titers were 11280 and 1 5120 respectively on 10 d, 30 d.</p><p><b>CONCLUSION</b>We expressed soluble HA with good bio-activity, which contributed to research on immune diagnosis, subunit vaccine, and monoclonal Abs for influenza.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Solubility / Virology / Gene Expression / Cell Line / Chemistry / Blotting, Western / Hemagglutinin Glycoproteins, Influenza Virus / Drosophila / Influenza, Human / Influenza A Virus, H1N1 Subtype Limits: Animals / Female / Humans Language: Chinese Journal: Chinese Journal of Experimental and Clinical Virology Year: 2013 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Solubility / Virology / Gene Expression / Cell Line / Chemistry / Blotting, Western / Hemagglutinin Glycoproteins, Influenza Virus / Drosophila / Influenza, Human / Influenza A Virus, H1N1 Subtype Limits: Animals / Female / Humans Language: Chinese Journal: Chinese Journal of Experimental and Clinical Virology Year: 2013 Type: Article