Study of polymerizing hemoglobin on cation exchange chromatography / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 1146-1149, 2008.
Article
in Zh
| WPRIM
| ID: wpr-318196
Responsible library:
WPRO
ABSTRACT
Poly-hemoglobin is the active component of hemoglobin-based blood substitutes. The excess reactivity of glutaraldehyde with hemoglobin in solution leads to poly-hemoglobin of a wide molecular weight distribution and a high average molecular weight. A new polymerization method has been tested to decrease the molecular weight distribution and the average molecular weight. The poly-hemoglobin with lower degree of modification (polymerization) was found enriched on the cation exchange columns and further polymerized with glutaraldehyde. The poly-hemoglobin of narrower molecular weight distribution has been prepared in this way.
Full text:
1
Index:
WPRIM
Main subject:
Pyridoxal Phosphate
/
Blood Substitutes
/
Hemoglobins
/
Chemistry
/
Chromatography, Ion Exchange
/
Methods
Limits:
Humans
Language:
Zh
Journal:
Journal of Biomedical Engineering
Year:
2008
Type:
Article