Determination of naringenin in Dendrobium officinale by HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To explore a characteristic chemical marker of Dendrobium officinale, establish determination method of its content and determine the naringenin content in D. officinale from different sources and growth years.</p><p><b>METHOD</b>The content of naringenin was determined by HPLC. HPLC analysis was made on a XB -C18 (4.6 mm x 250 mm, 5 microm) with methanol and water containing 0.2% phosphoric acid as mobile phase. The detection wavelength was 290 nm.</p><p><b>RESULT</b>The HPLC method showed good linearity within the range of 0.026-0.208 microg (r = 1). The average recovery of naringenin was 96.3% (RSD 1.8%). The naringenin content was the highest in 3 years D. officinale and had some differences from different sources.</p><p><b>CONCLUSION</b>The method is accurate and reliable. It is appropriate for the quantitative determination of naringenin in D. officinale and it's production.</p>