Gene expression of breast cancer resistance protein in adult acute lymphocytic leukemia and its clinical significance / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 31-34, 2008.
Article
in Zh
| WPRIM
| ID: wpr-318722
Responsible library:
WPRO
ABSTRACT
The objective of this study was to investigate the relationship between the expressions of breast cancer resistance protein (BCRP) gene and drug resistance as well as prognosis in adult patients with acute lymphocytic leukemia (ALL). Semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect the expression of BCRP gene in 97 adult patients with acute lymphocytic leukemia (ALL) and 30 normal subjects. Beta(2) microglobulin (beta(2)-MG) was used as an internal reference. BCRP/beta(2)-MG ratio >or= 0.48 was defined as BCRP positive. The results showed that the positive ratio of BCRP gene expression in untreated group was 28.7%, in contrast that in refractory and relapsed patients was 51.2%. The first complete remission rates in patients with negative and positive expression were 71.7% and 29.7% respectively. In ALL subtypes BCRP gene expression of L3 type was distinctly higher than that of L(1), L(2) types. In immune types the BCRP gene expression of B-ALL was higher than that of T cell type, especially in mature B cell type with obviously statistical significance (p<0.01). It is concluded that the high expression of BCRP gene may induce clinical drug resistance, and may be an unfavorable factor for prognosis in adult patients with acute lymphocytic leukemia.
Full text:
1
Index:
WPRIM
Main subject:
Prognosis
/
ATP-Binding Cassette Transporters
/
Drug Resistance, Neoplasm
/
Reverse Transcriptase Polymerase Chain Reaction
/
Precursor Cell Lymphoblastic Leukemia-Lymphoma
/
ATP Binding Cassette Transporter, Subfamily G, Member 2
/
Genetics
/
Metabolism
/
Neoplasm Proteins
Type of study:
Prognostic_studies
Limits:
Adolescent
/
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2008
Type:
Article