Expression of Rho-kinase and heme oxygenase in the corpus cavernosum of spontaneous hypertensive rats / 中华男科学杂志
National Journal of Andrology
; (12): 215-219, 2008.
Article
in Zh
| WPRIM
| ID: wpr-319243
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of Rho-kinase and heme oxygenase and the interaction among NOS/NO, HO/CO and RhoA/Rho-kinase in the corpus cavernosum of spontaneous hypertensive rats (SHRs).</p><p><b>METHODS</b>A series of electric stimuli were applied to the corpus cavernosum nerves of 7 SHRs, the changes of ICP/MAP observed continuously and the expressions of ROCK2, HO-2 and eNOS analyzed by Western blot and immunohistochemistry. Another 7 WKY rats were taken as controls.</p><p><b>RESULTS</b>Compared with the controls, ICP/MAP did not rise obviously (P < 0.05), HO-2 dropped sharply (P = 0.006) and the expression of the ROCK2 protein was elevated markedly (P = 0.017) in the SHRs. Immunohistochemistry showed HO-2 to be distributed in the smooth muscle cells and nervous cells of the corpus cavernosum, and eNOS mainly in the vascular endothelial cells. The expressions of HO-2 and eNOS were obviously reduced in the SHRs.</p><p><b>CONCLUSION</b>NOS/NO, HO/CO and RhoA/Rho-kinase are related with erectile dysfunction in SHRs and may interact on one another.</p>
Full text:
1
Index:
WPRIM
Main subject:
Penis
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Rats, Inbred SHR
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Rats, Inbred WKY
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Immunohistochemistry
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Blotting, Western
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Rho-Associated Kinases
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Heme Oxygenase (Decyclizing)
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Hypertension
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Erectile Dysfunction
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Metabolism
Limits:
Animals
Language:
Zh
Journal:
National Journal of Andrology
Year:
2008
Type:
Article