MAPK-ERK1/2 signaling pathway regulates osteogenic gene expression in rat osteoblasts in vitro / 南方医科大学学报
Journal of Southern Medical University
; (12): 1432-1436, 2013.
Article
in Zh
| WPRIM
| ID: wpr-319396
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of inhibition and activation of MAPK-ERK1/2 pathway on the expression of osteogenic genes and proliferation of rat osteoblasts in vitro.</p><p><b>METHODS</b>Primarily cultured rat osteoblasts, identified by cell morphology studies and ALP staining, were exposed to 1% or 5% rat serum for 24 h or to the specific MAPK-ERK1/2 inhibitor PD0325901. The downstream molecules of MAPK-ERK1/2 pathway including p-ERK1/2 and ERK1/2, osteogenic genes such as Runx2 and Type I collagen, and proliferating cell nuclear antigen (PCNA) were detected by Western Blotting, and alkaline phosphatase activities were analyzed quantitatively.</p><p><b>RESULTS</b>Compared with 1% rat serum-treated cells, exposure of the cells to a higher concentration (5%) of rat serum caused a significantly increased phosphorylation level of p-ERK1/2 (P<0.05) and obviously enhanced expressions of the osteogenic genes (Runx2, type I collagen and ALP) and PCNA (P<0.05). Inhibition of the MAPK-ERK1/2 pathway with PD0325901 resulted in suppressed expressions of the osteogenic genes and PCNA.</p><p><b>CONCLUSION</b>The activation of MAPK-ERK1/2 pathway promotes the expression of osteogenic genes such as Runx2, type I collagen and ALP and enhances the proliferative activity of the osteoblasts, while inhibition of this pathway suppresses the expressions of these genes and the cell proliferation, suggesting that this pathway may potentially serve as a therapeutic target for osteoporosis.</p>
Full text:
1
Index:
WPRIM
Main subject:
Osteoblasts
/
Pharmacology
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Phosphorylation
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Benzamides
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Cells, Cultured
/
Gene Expression Regulation
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Rats, Sprague-Dawley
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Proliferating Cell Nuclear Antigen
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Mitogen-Activated Protein Kinase 1
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MAP Kinase Signaling System
Type of study:
Prognostic_studies
Limits:
Animals
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2013
Type:
Article