Screening of plasma tumor biomarkers for hypopharyngeal carcinoma by proteomics method / 南方医科大学学报
Journal of Southern Medical University
; (12): 1173-1176, 2013.
Article
in Zh
| WPRIM
| ID: wpr-319453
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To screen tumor biomarkers in the plasma close related with hypopharyngeal carcinoma.</p><p><b>METHODS</b>Pooled plasma from 6 patients with hypopharyngeal carcinoma and 6 healthy individuals were collected. After removal of high-abundance plasma proteins, two-dimensional gel electrophoresis (2-DE) was performed to isolate the total proteins, and the protein spots with more than 2-fold differential expressions were detected by 2D analysis software followed by identification by MALDI-TOF/TOF mass spectrometer. Western blotting was performed to validate the expression level of α2-HS-glycoprotein.</p><p><b>RESULTS</b>A total of 11 differentially expressed protein spots were selected, including 5 upregulated proteins and 6 downregulated proteins. MALDI-TOF/TOF identified the upregulated proteins in hypopharyngeal carcinoma patients as alpha-2-HS-glycoprotein and haptoglobin and downregulated ones as Ig kappa chain C region and apolipoprotein A-I. Western blotting demonstrated that α-2-HS- glycoprotein expression level was consistent with that detected by 2-DE.</p><p><b>CONCLUSION</b>Patients with hypopharyngeal carcinoma show different plasma protein profiles from healthy individuals. These differentially expressed proteins may serve as potential specific tumor biomarkers for hypopharyngeal carcinoma.</p>
Full text:
1
Index:
WPRIM
Main subject:
Blood
/
Blood Proteins
/
Hypopharyngeal Neoplasms
/
Biomarkers, Tumor
/
Proteomics
/
Metabolism
/
Methods
Type of study:
Diagnostic_studies
/
Screening_studies
Limits:
Humans
/
Male
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2013
Type:
Article