An LC-MS/MS method for the simultaneous determination of amygdalin and paeoniflorin in human urine and application to urinary excretion study / 药学学报
Acta Pharmaceutica Sinica
; (12): 1330-1335, 2015.
Article
in Zh
| WPRIM
| ID: wpr-320081
Responsible library:
WPRO
ABSTRACT
The study aims to develop an LC-MS/MS method for the simultaneous determination of amygdalin and paeoniflorin in urine samples, and to investigate their urinary excretion characteristics in healthy volunteers after intravenous infusion administration of Huoxue-Tongluo lyophilized powder for injection (HTLPI). The urine samples were extracted by methanol, and then separated on a Hedera ODS-2 column with a mobile phase of acetonitrile and 5 mmol · L(-1) ammonium acetate buffer solution containing 0.05% formic acid (2080). Electrospray ionization source was applied and operated in the positive ion mode using MRM. The method exhibited good linearity over the concentration range of 0.03 -40 µg · mL(-1). The values on both the occasions (intra- and inter-day) were all within 15% at three concentration levels. No matrix effect and carry-over effect were observed. Amygdalin and paeoniflorin were stable in human urine under different storage conditions. Approximately 79.6% of the administered amount of amygdalin was excreted unchanged in urine within 24 h and which was 48.4% for paeoniflorin. The developed LC-MS/MS method can be applied to evaluate the urinary excretion of amygdalin and paeoniflorin.
Full text:
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Index:
WPRIM
Main subject:
Urine
/
Drugs, Chinese Herbal
/
Chromatography, Liquid
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Monoterpenes
/
Tandem Mass Spectrometry
/
Glucosides
/
Amygdalin
Limits:
Humans
Language:
Zh
Journal:
Acta Pharmaceutica Sinica
Year:
2015
Type:
Article