Molecular cloning of human sperm surface protein P34H gene and semi-quantitative analysis of its expression in testis and epididymidis / 中华男科学杂志

ABSTRACT

<p><b>OBJECTIVES</b>To identify, clone and sequence human sperm protein P34H gene and perform semi-quantitative analysis of its expression in testis and epididymidis.</p><p><b>METHODS</b>Total RNA was prepared from human epididymidis tissue. cDNA fragment encoding human P34H was amplified by RT-PCR using specific primers, and then was cloned into pGEM-T vector. Inserted P34H gene was sequenced by ABI 377 DNA Sequencer. Meanwhile, semi-quantitative analysis of P34H expression in testis and caput, corpus, cauda epididymidis was done by RT-PCR based on beta-actin as an inner control.</p><p><b>RESULTS</b>Human sperm protein P34H gene was successfully cloned and its cDNA sequence was submitted to GenBank(Accession No. AF515625).</p><p><b>CONCLUSIONS</b>The cloning of P34H gene may be useful for the further basic and clinical studies on P34H.</p>