Effect of homoharringtonine on expression of NF-κB and BCL-2 proteins in K562 cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 78-81, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-325208
ABSTRACT
This study was aimed to investigate the effect of homoharringtonine (HHT) on K562 cell proliferation, apoptosis and expression of BCL-2 and NF-κB proteins. The cells proliferation was assayed with MTT method, the cell apoptosis, cell cycle and BCL-2 expression were analyzed with flow cytometry, NF-κB protein expression was detected with Western blot. The results showed that HHT concentration-dependently inhibited proliferation of K562 cells, the IC50 at 48 h was 43.89 ng/ml. Treated with HHT 10 ng/ml for 48 h, K562 cell apoptosis significantly increased, cell cycle was blocked at G0/G1, the expression level of BCL-2 and NF-κB proteins was lower than that in control group (P < 0.05). It is concluded that HHT may inhibit the proliferation of K562 cells, and down-regulating expression levels of BCL-2 and NF-κB may be one of its anti-CML mechanisms.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
NF-kappa B
/
Proto-Oncogene Proteins c-bcl-2
/
K562 Cells
/
Flow Cytometry
/
Harringtonines
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2013
Type:
Article
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