Construction of the nucleic vaccine pVVP3L-18HN and its antitumor effect on human laryngeal carcinoma / 中华耳鼻咽喉头颈外科杂志
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
;
(12): 566-570, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-325323
ABSTRACT
<p><b>OBJECTIVE</b>Nucleic vaccine of pVVP3IL-18HN expressing apoptin gene, Newcastle disease virus HN gene and IL-18 gene were constructed to observe the combinative antitumor effect of the above three genes.</p><p><b>METHODS</b>Eukaryotic expression plasmid pVVP3IL-18HN was constructed by inserting apoptin gene and fragment comprising fused IL-18HN gene and IRES promoter into the downstream of CMV promoter of vector pVAX1. The expression of inserted gene was identified by RT-PCR, indirect immunofluorescence and Western-blot. The recombinant plasmid was introduced into Hep-2 cells by liposome, then suppression rate of Hep-2 of different time and different quantity was calculated according to MTT results.</p><p><b>RESULT</b>The recombinant plasmid of pVVP3IL-18HN suppressed Hep-2 successfully and its suppression rate was up to 61.9% with 20 microg/ml, incubation of 72 hours.</p><p><b>CONCLUSION</b>The nucleic vaccine constructed pVVP3IL-18HN had antitumor effect on Hep-2. It may can be used to the therapy and research of laryngeal carcinoma.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Newcastle disease virus
/
Transfection
/
Gene Expression
/
HN Protein
/
Laryngeal Neoplasms
/
Cancer Vaccines
/
Vaccines, DNA
/
Interleukin-18
/
Allergy and Immunology
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
Year:
2005
Type:
Article
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