Preparation and identification of a single-chain antibody fragment against high pathogenic H5N1 avian influenza virus / 生物工程学报
Chinese Journal of Biotechnology
; (12): 292-296, 2007.
Article
in Zh
| WPRIM
| ID: wpr-325377
Responsible library:
WPRO
ABSTRACT
Previously, an mAb 10F7 was developed against H5N1 hemagglutinin, which was highly specific to 34 different H5N1 strains and showed good neutralizing activity. In the present study, the single-chain fragment of the antibody was cloned into a prokaryotic vector and then expressed in E. coli. The activity of the scFv was tested in hemagglution inhibition and neutralization experiment. Two H5N1 virus strains were inhibited to bind erythrocyte cells by the scFv while the H9 virus was not. Also, five H5N1 virus strains were neutralized during infecting MDCK cells. These results showed an approachable method for developing therapeutic antibody to H5N1 virus.
Full text:
1
Index:
WPRIM
Main subject:
Virology
/
Birds
/
Recombinant Proteins
/
Neutralization Tests
/
Cell Line
/
Amino Acid Sequence
/
Cell Line, Tumor
/
Electrophoresis, Polyacrylamide Gel
/
Allergy and Immunology
/
Influenza A Virus, H5N1 Subtype
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2007
Type:
Article