A nested multiplex PCR assay for rapid subtyping the prevailing HIV-1 strains in Guangxi / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 482-484, 2009.
Article
in Zh
| WPRIM
| ID: wpr-325504
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a rapid nested multiplex PCR assay for subtyping HIV-1 CRF01_AE, CRF07_BC, CRF08_BC, B, and C strains prevailing in Guangxi.</p><p><b>METHOD</b>Subtype-specific primers were designed for these subtypes based on their gag sequences. The subtypes of HIV-1 samples from Guangxi were determined by nested multiplex PCR and DNA sequencing and phylogenetic analysis, respectively, and then the sensitivity and the specificity of nested multiplex PCR were calculated.</p><p><b>RESULTS</b>Nested multiplex PCR could correctly classify the 5 known-subtype samples, and were not reactive to all HIV-negative samples. Of the 72 HIV-positive samples, 66 were correctly identified as CRF01_AE, CRF07_BC, CRF08_BC, and B by this assay, giving a sensitivity of 91.7% (66/72), and a specificity of 100%.</p><p><b>CONCLUSION</b>This assay is a simple, fast, and cost-effective subtyping method for HIV-1 CRF01-AE, CRF07_BC, CRF08_BC, and B strains prevailing in Guangxi.</p>
Full text:
1
Index:
WPRIM
Main subject:
Phylogeny
/
Virology
/
Molecular Sequence Data
/
HIV Infections
/
China
/
Polymerase Chain Reaction
/
HIV-1
/
Classification
/
DNA Primers
/
Economics
Type of study:
Health_economic_evaluation
/
Prognostic_studies
Limits:
Humans
Country/Region as subject:
Asia
Language:
Zh
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2009
Type:
Article