Prevention of bone loss by injection of insulin-like growth factor-1 after sciatic neurectomy in rats / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
; (6): 158-162, 2013.
Article
in En
| WPRIM
| ID: wpr-325720
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>Injection of insulin-like growth factor-1 (IGF-1) can prevent bone loss in sciatic nerve transaction rats. We try to investigate the action mechanism of IGF-1 on bone formation.</p><p><b>METHODS</b>A total of 40 adult male Spragne-Dawley rats were divided into two groups (experimental group and control group) with 20 animals in each. Sciatic neurectomy was performed to model disuse osteoporosis in all rats. IGF-1 was administered in experimental group with the dose of 100 microgramme/kilogram per day for 3 days. Meanwhile, the rats in control group were treated with saline. Bone mineral density was measured by dual-energy X-ray absorptiometry 4 and 6 weeks after neurectomy respectively. Expression of Osterix and Runx2 was determined by reverse transcription-polymerase chain reaction (RT-PCR) assay.</p><p><b>RESULTS</b>There was a significant increase in the bone mineral density of experimental group compared with control group. There was a significant decrease in the level of receptor activator of nuclear factor-kappaB-ligand but an increase in the level of osteoprotegerin 4 and 6 weeks after neurectomy in the experimental group compared with control one. The expression of Osterix and Runx2 was up-regulated in the bone marrow of experimental group compared with control group.</p><p><b>CONCLUSION</b>IGF-1 can increase bone formation by stimulation of osteoblast number and activity, and reduce bone resorption by restriction of differentiation of osteoclast, suggesting that IGF-1 may improve the therapeutic efficacy for disuse osteoporosis.</p>
Full text:
1
Index:
WPRIM
Main subject:
Osteoblasts
/
Physiology
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Sciatic Nerve
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General Surgery
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Transcription Factors
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Bone Resorption
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Insulin-Like Growth Factor I
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Immunohistochemistry
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Bone Density
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Cell Differentiation
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Chinese Journal of Traumatology
Year:
2013
Type:
Article