Study on mechanism of inducing apoptosis in human hepatoma SMMC-7721 cells by DMC, a chalcone from buds of Cleistocalyx operculatus / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2942-2946, 2014.
Article
in Zh
| WPRIM
| ID: wpr-327863
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the in-vitro inducing apoptosis mechanism of human hepatoma SMMC-7721 cells by 2',4'-di- hydroxy-6'-methoxy-3',5'-dimethylchalcone (DMC), a chalcone compound from Cleistocalyx operculatus.</p><p><b>METHOD</b>Quantitative DNA fragmentation assay was carried out to detect the effect of DMC of different concentrations on SMMC-7721 cells, according to the method of Sellins and Cohen with some modifications. Telomerase activities of the cells were determined by PCR-ELISA methods. The expression quantity of c-myc and hTERT mRNA were determined by semi-quantitative RT-PCR The effect of DMC on expression levels of cmyc and hTERT protein were measured by western blot.</p><p><b>RESULT</b>The percentage of DNA fragmentation increased with notable concen- tration dependence, after treatment with DMC for 48 h. Compared with that of control group, the telomerase activity of the cells de- creased by (66.2 ± 2.1)% after 48 h treatment with 20 μmol x L(-1) DMC, the mRNA expression of c-myc and hTERT decreased by (67.3 ± 2.1)% and (64.4 ± 2.3)%, respectively, and the protein expression of c-myc and hTERT decreased by (69.6 ± 1.9)% and (71.3 ± 2.4)%, respectively.</p><p><b>CONCLUSION</b>DMC can induce SMMC-7721 cell apoptosis and the apoptosis mechanism may be related to the decreased mRNA and protein expression of c-myc and hTERT.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
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Pharmacology
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RNA, Messenger
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Chemistry
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Proto-Oncogene Proteins c-myc
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Apoptosis
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Carcinoma, Hepatocellular
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Telomerase
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Syzygium
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Cell Line, Tumor
Limits:
Humans
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2014
Type:
Article