Effect of diallyl disulfide on the expression and secretion of VEGF in HL-60 cells / 中华血液学杂志
Chinese Journal of Hematology
; (12): 626-629, 2006.
Article
in Zh
| WPRIM
| ID: wpr-328408
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the proliferation inhibition of human leukemic cell line HL-60 and the expression of vascular endothelial growth factor (VEGF) mRNA and secretion of VEGF protein in HL-60 cells treated with diallyl disulfide (DADS).</p><p><b>METHODS</b>MTT was used to test the cell growth, semi-quantitative RT-PCR and ELISA to study the expression of VEGF mRNA and secretion of VEGF protein.</p><p><b>RESULTS</b>DADS significantly inhibited proliferation of HL-60 cell and the inhibiting effects showed a dose (r > 0.9, P < 0.01) and time-dependent( r > 0.7, P < 0.01) manner. The expression of VEGF mRNA and secretion of VEGF protein could be down regulated by 0.625, 1.250, and 2.500 microg/mL DADS in HL-60 cells for 24,48 and 72 hours exposure and the effects also showed dose -dependence(r > 0.9, P < 0.01). The growth inhibition rates of DADS in HL-60 cells at three dosages for 24 hours were (8.19 +/- 3.34)%, (16.79 +/- 2.07)% and (21.30 +/- 2.72)%, those for 48 hours were (11.93 +/- 3.93)%, (22.81 +/- 2.31)% and (30.74 +/- 2.03)%, for 72 hours were (16.68 +/- 2.37)%, (28.54 +/- 3.26)% and (36.59 +/- 2.37)% respectively, The difference between the DADS-treated and untreated HL-60 cells was statistically significant (P < 0.01). There were also statistically significant differences among the three groups of different dosages (P < 0.01).</p><p><b>CONCLUSION</b>DADS can effectively inhibit the proliferation of HL-60 cells. DADS probably exerts its anti-leukemia effects by reducing the expression of VEGF mRNA and secretion of VEGF protein in HL-60 cells.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
RNA, Messenger
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HL-60 Cells
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Bodily Secretions
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Reverse Transcriptase Polymerase Chain Reaction
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Vascular Endothelial Growth Factor A
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Disulfides
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Dose-Response Relationship, Drug
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Allyl Compounds
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Antineoplastic Agents
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Hematology
Year:
2006
Type:
Article