Antiviral effects of aqueous extract from Spatholobus suberectus Dunn. against coxsackievirus B3 in mice / 中国结合医学杂志
Chin. j. integr. med
; Chin. j. integr. med;(12): 764-769, 2011.
Article
in En
| WPRIM
| ID: wpr-328475
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the antiviral effects of the aqueous extract of Spatholobus suberectus Dunn. (A.E.), a Chinese medicinal herb, against coxsackievirus B3 (CVB3).</p><p><b>METHODS</b>The antiviral effects of A.E. against CVB3 in vitro (primarily cultured myocardial cells) and in vivo (BALB/c mice) were determined. Serum pharmacological method was also adopted by in vitro experiments. The effects of A.E. inhibiting the CVB3 mRNA expression were compared by RT-PCR in mice in vivo.</p><p><b>RESULTS</b>A.E. exhibited obvious antiviral: effects in vivo, and serum samples obtained from the rats with oral administration of A.E. (10 μg/mL, 5 μg/mL), reduced the virus titers in the infected myocardial cells (3.00±0.70, 3.55±0.52, P<0.01). Meanwhile, the viral myocarditis induced by CVB3 was inhibited significantly by A.E., and the 15-day mortality was reduced to 40% and 45% (P<0.01) in mice treated with A.E. at doses of 50 mg/kg and 100 mg/kg, respectively, while the 30-day mortality was decreased to 45% and 50%, respectively (P<0.01). Moreover, the mRNA expression of Coxsackie virus B3 was significantly inhibited by A.E.</p><p><b>CONCLUSION</b>Aqueous extract of Spatholobus suberectus Dunn. (A.E.) has inhibitory effect on CVB3 both in vitro and in vivo.</p>
Full text:
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Index:
WPRIM
Main subject:
Antiviral Agents
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Organ Size
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Pathology
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Pharmacology
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Virology
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Blood
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Body Weight
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Vero Cells
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RNA, Messenger
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Plant Extracts
Limits:
Animals
Language:
En
Journal:
Chin. j. integr. med
Year:
2011
Type:
Article