Effect of CD133(+) cells on the radiosensitivity of rectal cancer cells / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 103-108, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-328974
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of CD133(+) cells on radiosensitivity of rectal cancer cells.</p><p><b>METHODS</b>In vitro experiments CD133(+) cells were purified with Immunomagnetic beads from human rectal cancer cell line SW480 cells and annexin V/PI staining was used to determine apoptosis in CD133(+) and CD133(-) cells. In vivo experiments Transplanted rectal tumor was established in 30 nude mice using primarily established SW480 cells. The tumor cells were divided into CD133-high and CD133-low groups based on the immunohistochemical staining of CD133 expression of the tumor cells. The tumor size after irradiation was recorded every three days.</p><p><b>RESULTS</b>CD133(+) cells had a much lower percentage of apoptosis after radiation exposure compared with CD133(-) cells [(12.6 ± 3.2) % vs. (38.8 ± 6.7) %, P < 0.01]. In vivo experiment showed that the normalized tumor size of CD133-high group (3.00 ± 0.32) became significantly larger than that of the CD133-low group(2.55 ± 0.29) at the ninth day and this difference lasted until the observation end (P < 0.05).</p><p><b>CONCLUSIONS</b>CD133(+) cells have a radioresistant effect on rectal cancer cells and may become a potential therapeutic target in the radiotherapy of rectal cancer.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Peptides
/
Radiation Effects
/
Radiation Tolerance
/
Radiotherapy
/
Rectal Neoplasms
/
Glycoproteins
/
Adenocarcinoma
/
Random Allocation
/
Antigens, CD
Type of study:
Controlled clinical trial
Limits:
Animals
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2014
Type:
Article
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