Acidic HEPES-KH reperfusion enhances myocardial protection in immature rabbits / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
; (6): 107-108, 2002.
Article
in En
| WPRIM
| ID: wpr-329170
Responsible library:
WPRO
ABSTRACT
To study the effects of different pH HEPES-KH reperfusate solution on immature myocardial protection, isolated perfused Langendorff model from immature rabbit hearts were developed formed. Control group (C) was perfused only with pH 7.4 HEPES-KH solution for 90 min. Ischemia/reperfusion group (group I/R) was perfused with pH 7.4 HEPES-KH solution before ischemia or after ischemia. Experimental group (group E), after ischemia, was perfused with pH 6.8, pH 7.1 and pH 7.4 HEPES-KH solutions for 5 min, 5 min, and 20 min, respectively. The left ventricular function recovery, MWC, LDH and CK leakage, MDA, ATP content, and SOD activity were determined. Our results showed that the left ventricular function recovery, ATP content and SOD activity in group E were higher than those of group I/R (P < 0.05). MWC, MDA content, LDH and CK leakage in group E were lower than those of group I/R (P < 0.05). These findings suggested that pH paradox might be one of important mechanisms for immature myocardial ischemia-reperfusion injury, and acidic perfusate, at the beginning of reperfusion, might attenuate pH paradox and ameliorate functional recovery in isolated perfused immature rabbit hearts.
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Superoxide Dismutase
/
Cardioplegic Solutions
/
Myocardial Reperfusion Injury
/
Random Allocation
/
Myocardial Ischemia
/
Heart
/
Heart Arrest, Induced
/
Hydrogen-Ion Concentration
/
Metabolism
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Year:
2002
Type:
Article