Lentivirus-mediated shRNA interference of Cx26 suppresses epithelial mesenchymal transition and invasion of highly invasive hepatocellular carcinoma cells in vitro / 南方医科大学学报
Journal of Southern Medical University
; (12): 1743-1747, 2014.
Article
in Zh
| WPRIM
| ID: wpr-329209
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of lentivirus-mediated shRNA interference of Cx26 on epithelial-mesenchymal transition (EMT) and invasion of highly invasive human hepatocellular carcinoma cells in vitro.</p><p><b>METHODS</b>SK-Hep-1 cells were infected with the lentivirus for delivering Cx26 shRNA, and the stably transfected cells were selected by puromycin. The interference efficiency of shRNA-Cx26 was assessed with real-time PCR and Western blotting. The morphological changes of the transfected SK-Hep-1 cells were observed microscopically, and the protein expressions of E-cadherin and vimentin were detected using Western blotting. The effect of Cx26 interference on the invasiveness of SK-Hep-1 cells was determined by Transwell invasion assay.</p><p><b>RESULTS</b>Compared with SK-Hep-1 cells infected with empty EGFP vector and uninfected cells, the cells transfected with shRNA-Cx26 showed significantly reduced mRNA and protein expressions of Cx26 (P<0.01), which resulted in obvious morphological conversion from mesenchymal cells to epithelial cells. shRNA-Cx26-transfected cells showed significantly increased E-cadherin protein expression (P<0.01) but decreased vimentin expression (P<0.01) with obviously attenuated invasive ability in vitro (P<0.01).</p><p><b>CONCLUSION</b>Targeted down-regulation of Cx26 expression can inhibit the EMT and invasion of SK-Hep-1 cells in vitro.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
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Vimentin
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RNA, Messenger
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Transfection
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Cadherins
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Down-Regulation
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Connexins
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Carcinoma, Hepatocellular
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Lentivirus
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RNA, Small Interfering
Limits:
Humans
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2014
Type:
Article