Preliminary screening of downstream proteins of Sox2 and role of Sox2 in colonic cancer cell migration and invasion / 南方医科大学学报
Journal of Southern Medical University
; (12): 1594-1600, 2014.
Article
in Zh
| WPRIM
| ID: wpr-329240
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To screen the down-stream proteins of transcription factor Sox2 and explore the role of Sox2 in the proliferation and migration of colonic cancer cells in vitro.</p><p><b>METHODS</b>The cellular proteins were separated by SDS-PAGE electrophoresis and stained with Coomassie blue and amine plated silver. The differentially expressed proteins was identified by mass spectrometry and verified by QPCR and Western blotting. A cell counting kit-8 (CCK8) assay was performed to evaluate the cell proliferation, and the cell migration was assessed using Transwell assay.</p><p><b>RESULTS</b>S3a was identified by proteomics technology as a Sox2-downregulated protein while ENO1 and gama-actin the up-regulated proteins. QPCR and Western blotting analyses showed that overexpression of Sox2 significantly decreased the expression of S3a (P<0.005) and increased the expression of ENO1(P<0.05), but had no significant effect on gama-actin expression. Sox2 overexpression obviously promoted cell proliferation and migration (P<0.05), while inhibition of Sox2 produced contrary effects (P<0.05).</p><p><b>CONCLUSION</b>Sox2 negatively regulates S3a expression and positively regulates ENO1 expression to promot the proliferation and migration of colonic cancer cells.</p>
Full text:
1
Index:
WPRIM
Main subject:
Ribosomal Proteins
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Down-Regulation
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Up-Regulation
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Cell Movement
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Colonic Neoplasms
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Proteomics
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Cell Line, Tumor
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Cell Proliferation
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SOXB1 Transcription Factors
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Metabolism
Type of study:
Diagnostic_studies
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Prognostic_studies
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Screening_studies
Limits:
Humans
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2014
Type:
Article