Anti-tumor effects of pNEgr-mIL-12 recombinant plasmid induced by X-irradiation and its mechanisms / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 135-143, 2004.
Article
in English
| WPRIM
| ID: wpr-329648
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of gene radiotherapy combining injection of recombinant plasmid pNEgr-mIL-12 with local X-irradiation on cancer growth and to elucidate the mechanisms of tumor inhibition.</p><p><b>METHODS</b>Alkaline lysis was used to extract the plasmid and polyethylene glycol 8000 (PEG 8000) was applied for further purification of plasmids. Enzyme-linked immunosorbent assay (ELISA) was used to detect the expression of IL-12 protein. C57BL/6J mice were subcutaneously inoculated with B16 melanoma cells and the plasmid was injected directly into the tumor. Gene-radiotherapy combining pNEgr-mIL-12 recombinant plasmid with X-irradiation was given three times to C57BL/6J mice bearing B16 melanoma. Changes in immunologic parameters of tumor-bearing mice were detected with relevant immunologic assays.</p><p><b>RESULTS</b>Results showed a significant decrease in tumor growth rate (P<0.05-0.001) after 3 times of gene-radiotherapy with IL-12 and X-irradiation. Immunologic studies showed a significant increase in CTL and NK cytolytic activity (P<0.05-0.001) and an up-regulated secretion of IFN-gamma and TNF-alpha (P<0.01-0.001). Moreover, the expression of mIL-12 in B16 melanoma cells of the treated tumor-bearing mice was found to be higher than that of control.</p><p><b>CONCLUSION</b>pNEgr-mIL-12 plasmid combined with X-irradiation can increase tumor control and the mechanism of increased tumor inhibition is related to the enhancement of anticancer immunity in tumor-bearing mice.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Radiotherapy
/
Spleen
/
Therapeutics
/
X-Rays
/
Melanoma, Experimental
/
Enzyme-Linked Immunosorbent Assay
/
Killer Cells, Natural
/
T-Lymphocytes, Cytotoxic
/
Genetic Therapy
Limits:
Animals
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2004
Type:
Article
Similar
MEDLINE
...
LILACS
LIS