The variation of PPAR pathway molecules in the lung tissue of rats under hyperbaric oxygen exposure / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 298-300, 2012.
Article
in Zh
| WPRIM
| ID: wpr-329882
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression pattern of peroxisome proliferator-activated receptor (PPAR) pathway molecules in rat lung tissue under hyperbaric oxygen exposure.</p><p><b>METHODS</b>Twenty seven male SD rats were randomly divided into hyperbaric normoxia group (0.23 MPa air), hyperbaric oxygen treatment time series group (0.23 MPa oxygen, were exposed for 2 h, 4 h, 6 h or 8 h), continuous small flow of ventilation to maintain cabin O2 concentration > 99%. HE staining of lung tissue morphological changes and application oligo microarray to each time point lung were observed. Part of the PPAR pathway genes were validated by RT-PCR.</p><p><b>RESULTS</b>Compared with hyperbaric normoxia group, the lung injury caused by hyperbaric oxygen treatment gradually deteriorated during the time series. Expression microarray analysis of gene ontology (Go) enrichment analysis results in a class of PPAR pathway class included multiple PPAR pathway molecule. RT-PCR results suggested that PPAR-8 and PPAR-Y were up-regulated in the lung tissue after a long time exposure to hyperbaric oxygen.</p><p><b>CONCLUSION</b>Pro-longed hyperbaric oxygen exposure causing pulmonary oxygen toxicity can induce the activation of the PPAR pathway.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
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Signal Transduction
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Rats, Sprague-Dawley
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Peroxisome Proliferator-Activated Receptors
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Hyperbaric Oxygenation
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Lung
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Metabolism
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Applied Physiology
Year:
2012
Type:
Article