In silico cloning and bioinformatics analysis of an AP2/EFR family gene from Arnebia euchroma / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2251-2257, 2014.
Article
in Zh
| WPRIM
| ID: wpr-330311
Responsible library:
WPRO
ABSTRACT
A cDNA sequence of Arnebia euchroma AP2/ERF named AeAP2/ERF was cloned by in silico cloning in this study, using ACX71873 sequence from Lithospermum erythrorhizon as the probe sequence. Some characters of the AP2/ERF gene and encoded protein sequences were predicted and analyzed by the bioinformatics methods, including general physical and chemical properties, hydrophobieity, signal peptide, secondary structure, localization sites in cells. Results showed that the 876 bp long gene included a 1 077 bp ORF and encoding 205 amino acid. The AeAP2/ERF protein had no signal peptide, it was a hydrophilic proteins located in nucleus. The function of the AP2/ERF protein was mainly involved with metabolism controlling and signal transduction.
Full text:
1
Index:
WPRIM
Main subject:
Phylogeny
/
Plant Proteins
/
Transcription Factors
/
Computer Simulation
/
Molecular Sequence Data
/
Base Sequence
/
Chemistry
/
Amino Acid Sequence
/
Classification
/
Cloning, Molecular
Type of study:
Prognostic_studies
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2014
Type:
Article