Identification of Placenta hominis and its adulterants using COI barcode / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2204-2207, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-330320
ABSTRACT
In order to provide a new method for the identification of Placenta hominis, the COI barcode has been employed to identify the P. hominis medicinal materials and its adulterants. Genomic DNA was extracted from the experimental samples. The COI sequences were amplified and sequenced bi-directionally. Sequence assembly and consensus sequence generation were performed using the CodonCode Aligner. NJ tree was constructed by MEGA6.0 software. COI sequences can be successfully obtained from all experimental samples. The intra-specific variation and inter-specific divergence were calculated. The average intra-specific K2P distance of P. hominis was 0.001 and the maximum intra-specific distance was 0.008. The cluster dendrogram constructed can be seen that the same genus is together, and distinguished from its adulterants. It is concluded that P. hominis and its adulterants can be correctly identified by DNA barcoding method.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phylogeny
/
Placenta
/
Quality Control
/
Swine
/
Sheep
/
Molecular Sequence Data
/
Chemistry
/
Drug Contamination
/
Electron Transport Complex IV
/
DNA Barcoding, Taxonomic
Type of study:
Diagnostic study
Limits:
Animals
/
Female
/
Humans
/
Pregnancy
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2014
Type:
Article
Similar
MEDLINE
...
LILACS
LIS